A central composite face-centered design was used to study and to optimize lead biosorption from aqueous solution on Aspergillus terreus biomass. Four factors such as stirring speed, temperature, solution pH and biomass dose at different levels were studied. The hierarchical quadratic model were established by adding replicates at the central point and axial points to the initial full factorial design (24). The percentage removal of lead was affected by biomass dose, pH, and interactions between pH and biomass dose, pH and stirring speed, pH and temperature. The hierarchical quadratic model described adequately the Response surface based on the adjusted determination coefficient (R2Adj=0.97) and the adequate precision ratio (42.21). According to this model, the optimal conditions to remove lead completely from aqueous solutions (at initial lead concentration of 50 mg/L and solutions of 100 mL) with Aspergillus terreus were at pH 5.2, 50oC, stirring speed of 102/min and a biomass dose of 139 mg. The Response surface methodology can be used to determine the optimal conditions for metal adsorption on several adsorbents. In addition, results reported in this research demonstrated the feasibility of employing A. terreus as biosorbent for lead removal.